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A Novel Cryogenic Preparation Technique fod Near-Instantaneous Vitrification of Biological Specimens
时间:2018-09-26 09:44    点击:   所属单位:空间科学与技术学院
讲座名称 A Novel Cryogenic Preparation Technique fod Near-Instantaneous Vitrification of Biological Specimens
讲座时间 2018-09-28 10:00:00
讲座地点 新校区G楼240报告厅
讲座人 谷静
讲座人介绍 I am an instructor at the Department of Electrical and Computer Engineering (ECE), National University of Singapore (NUS) since March 2017. My teaching curriculum is extensive and related to Computer Interfaces and Architecture, Analog and Digital Circuits and System, Electromagnetic and Signal Processing etc.
I received my PhD in ECE from NUS in 2017. My PhD research is on developing “a novel cryogenic preparation technique for near-instantaneous vitrification of biological specimens”. My Bachelor's degree is specialized in microelectronics, received in ECE of NUS as well.
讲座内容

The study of biological specimens with charged particles remains integral to the advancement of biological sciences owing to its superior resolution. Helium Ion Microscopy (HIM) is well known as a better imaging method comparing to conventional Scanning Electron Microscopy (SEM) in terms of higher resolution at high magnification, charge neutrality for insulating materials, and ultrastructure preservation without metal coating.

To preserve biological specimen in its dehydrated state and achieve microscopic imaging in a vacuum environment, cryogenic fixation is considered necessary and efficient specimen preparation process. However, the existing cryogenic fixation techniques present inevitable obstacles to capture faithful and reliable specimen’s ultrastructures due to limited vitreous depth. The study presented demonstrates a microwave assisted cryogenic fixation for biological vitrification enhancement. Theoretical analysis are carried out and shown that a pulsed microwave signal is effective to suppress the formation of water pentamers thus nucleation sites, minimizing crystal formation. An optimized jet freezing system integrated with efficient microwave irradiation applicator is established for in-situ optical evaluation of vitrification. The experimental results prove that the proposed cryogenic fixation technique effectively inhibit the ice crystallization.

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